Wednesday, October 27, 2021

WHAT IS GLOBAL WARMING? IT IS DEADLY!

Håkan Jansson/Alamy 

Q: What is global warming?

A: Since the Industrial Revolution, the global annual temperature has increased in total by a little more than 1 degree Celsius, or about 2 degrees Fahrenheit. Between 1880—the year that accurate recordkeeping began—and 1980, it rose on average by 0.07 degrees Celsius (0.13 degrees Fahrenheit) every 10 years. Since 1981, however, the rate of increase has more than doubled: For the last 40 years, we’ve seen the global annual temperature rise by 0.18 degrees Celsius, or 0.32 degrees Fahrenheit, per decade.

The result? A planet that has never been hotter. Nine of the 10 warmest years since 1880 have occurred since 2005—and the 5 warmest years on record have all occurred since 2015. Climate change deniers have argued that there has been a “pause” or a “slowdown” in rising global temperatures, but numerous studies, including a 2018 paper published in the journal Environmental Research Letters, have disproved this claim. The impacts of global warming are already harming peoplearound the world.

Now climate scientists have concluded that we must limit global warming to 1.5 degrees Celsius by 2040 if we are to avoid a future in which everyday life around the world is marked by its worst, most devastating effects: the extreme droughts, wildfires, floods, tropical storms, and other disasters that we refer to collectively as climate change. These effects are felt by all people in one way or another but are experienced most acutely by the underprivileged, the economically marginalized, and people of color, for whom climate change is often a key driver of poverty, displacement, hunger, and social unrest.

Q: What causes global warming?

A: Global warming occurs when carbon dioxide (CO2) and other air pollutants collect in the atmosphere and absorb sunlight and solar radiation that have bounced off the earth’s surface. Normally this radiation would escape into space, but these pollutants, which can last for years to centuries in the atmosphere, trap the heat and cause the planet to get hotter. These heat-trapping pollutants—specifically carbon dioxide, methane, nitrous oxide, water vapor, and synthetic fluorinated gases—are known as greenhouse gases, and their impact is called the greenhouse effect.

Though natural cycles and fluctuations have caused the earth’s climate to change several times over the last 800,000 years, our current era of global warming is directly attributable to human activity—specifically to our burning of fossil fuels such as coal, oil, gasoline, and natural gas, which results in the greenhouse effect. In the United States, the largest source of greenhouse gases is transportation (29 percent), followed closely by electricity production (28 percent) and industrial activity (22 percent).

Curbing dangerous climate change requires very deep cuts in emissions, as well as the use of alternatives to fossil fuels worldwide. The good news is that countries around the globe have formally committed—as part of the 2015 Paris Climate Agreement—to lower their emissions by setting new standards and crafting new policies to meet or even exceed those standards. The not-so-good news is that we’re not working fast enough. To avoid the worst impacts of climate change, scientists tell us that we need to reduce global carbon emissions by as much as 40 percent by 2030. For that to happen, the global community must take immediate, concrete steps: to decarbonize electricity generation by equitably transitioning from fossil fuel–based production to renewable energy sources like wind and solar; to electrify our cars and trucks; and to maximize energy efficiency in our buildings, appliances, and industries.

Q: How is global warming linked to extreme weather?

A: Scientists agree that the earth’s rising temperatures are fueling longer and hotter heat waves, more frequent droughts, heavier rainfall, and more powerful hurricanes.

In 2015, for example, scientists concluded that a lengthy drought in California—the state’s worst water shortage in 1,200 years—had been intensified by 15 to 20 percent by global warming. They also said the odds of similar droughts happening in the future had roughly doubled over the past century. And in 2016, the National Academies of Science, Engineering, and Medicine announced that we can now confidently attribute some extreme weather events, like heat waves, droughts, and heavy precipitation, directly to climate change.

The earth’s ocean temperatures are getting warmer, too—which means that tropical storms can pick up more energy. In other words, global warming has the ability to turn a category 3 storm into a more dangerous category 4 storm. In fact, scientists have found that the frequency of North Atlantic hurricanes has increased since the early 1980s, as has the number of storms that reach categories 4 and 5. The 2020 Atlantic hurricane season included a record-breaking 30 tropical storms, 6 major hurricanes, and 13 hurricanes altogether. With increased intensity come increased damage and death. The United States saw an unprecedented 22 weather and climate disastersthat caused at least a billion dollars’ worth of damage in 2020, but 2017 was the costliest on record and among the deadliest as well: Taken together, that year's tropical storms (including Hurricanes Harvey, Irma, and Maria) caused nearly $300 billion in damage and led to more than 3,300 fatalities.

The impacts of global warming are being felt everywhere. Extreme heat waves have caused tens of thousands of deaths around the world in recent years. And in an alarming sign of events to come, Antarctica has lost nearly four trillion metric tons of ice since the 1990s. The rate of loss could speed up if we keep burning fossil fuels at our current pace, some experts say, causing sea levels to rise several meters in the next 50 to 150 years and wreaking havoc on coastal communities worldwide.


Tuesday, October 26, 2021

The Pfizer vaccines worked for me!

The mRNA

Pfizer’s vaccine is the first on the market that consists of actual genetic information from a virus in the form of messenger RNA, or mRNA, a type of molecule whose usual job is to transport copies of genetic instructions around a cell to guide the assembly of proteins. Imagine an mRNA as a long ticker tape carrying instructions. It's fairly delicate stuff, and that's why Pfizer's vaccine needs to be kept at around -100 °F (-73 °C) until it's used.

The new vaccine, delivered as a shot in the arm muscle, contains an RNA sequence taken from the virus itself; it causes cells to manufacture the big “spike” protein of the coronavirus, which the pathogen uses to glom onto a person’s cells and gain entry. On its own, without the rest of the virus, the spike is pretty harmless. But your body still reacts to it. This is what leaves you immunized and ready to repel the real virus if it turns up.

The mRNA in the vaccine, to be sure, isn’t quite the same as the stuff in your body. That’s good, because a cell is full of defenses ready to chop up RNA, especially any that doesn’t belong there. To avoid that, what’s known as “modified nucleosides” have been substituted for some of the mRNA building blocks.

But Pfizer is holding back a little. The spike gene sequence can be tweaked in small ways for better performance, by means that include swapping letters. We don’t think Pfizer has said exactly what sequence it is using, or what modified nucleosides. That means the content of the shot may not be 100% public.

The lipids

The Pfizer vaccine, like one from Moderna, uses lipid nanoparticles to encase the RNA. The nanoparticles are, basically, tiny greasy spheres that protect the mRNA and help it slide inside cells.

These particles are probably around 100 nanometers across. Curiously, that’s about the same size as the coronavirus itself.

Pfizer says it uses four different lipids in a “defined ratio.” The lipid ALC-0315 is the primary ingredient in the formulation. That’s because it’s ionizable—it can be given a positive charge, and since the RNA has a negative one, they stick together. It's also a component that can cause side-effects or allergic reactions. The other lipids, one of which is the familiar molecule cholesterol, are “helpers” that give structural integrity to the nanoparticles or stop them from clumping. During manufacturing, the RNA and the lipids are stirred into a bubbly mix to form what the FDA describes as a “white to off-white” frozen liquid.

Salts

The Pfizer vaccine contains four salts, one of which is ordinary table salt. Together, these salts are better known as phosphate-buffered saline, or PBS, a very common ingredient that keeps the pH, or acidity, of the vaccine close to that of a person’s body. You’ll understand how important that is if you’ve ever squeezed lemon juice on a cut. Substances with the wrong acidity can injure cells or get quickly degraded.

Sugar

The vaccine includes plain old sugar, also called sucrose. It’s acting here as a cryoprotectant to safeguard the nanoparticles when they’re frozen and stop them from sticking together.

Saline solution

Before injection, the vaccine is mixed with water containing sodium chloride, or ordinary salt, just as many intravenously delivered drugs are. Again, the idea is that the injection should more or less match the salt content of the blood.

No preservatives

Pfizer makes a point of saying its mixture of lipid nanoparticles and mRNA is “preservative-free.” That’s because a preservative that’s been used in other vaccines, thimerosal (which contains mercury and is there to kill any bacteria that might contaminate a vial), has been at the center of worries around over whether vaccines cause autism. The US Centers for Disease Control says thimerosal is safe; despite that, its use is being phased out. There is no thimerosal—or any other preservative—in the Pfizer vaccine. No microchips, either.

The vaccine is still known by the code name BNT162b, but once it’s authorized, expect Pfizer to give it a new, commercial name that conveys something about what’s in it and what it promises for the world.

We thank the following people for explaining the vaccine ingredients: Jacob Becraft and Aalok Shah, Strand Therapeutics; Yizhou Dong, Ohio State University; Jason Underwood, Pacific Biosciences; Andrey Zarur, Greenlight Biosciences; Charles L. Cooney, MIT; and the communications staffs of Pfizer and Moderna Therapeutics.

By Antonio Regalato

Gladstone-UCSF Institute of Genomic Immunology Symposium

Inbox

Gladstone Events events@gladstone.ucsf.edu Unsubscribe




        

Forward to a Friend

GLADSTONE-UCSF INSTITUTE OF GENOMIC IMMUNOLOGY

Innovators in Genomic Immunology

November 3, 2021
9am–5pm PDT • Online

Join this 1-day symposium to meet the investigators of the Gladstone-UCSF Institute of Genomic Immunology and discover how their labs are using genomic and genome engineering technologies to address key questions in human immunology and repurpose the immune system to fight disease.

You’ll also get to hear from invited speaker Carl June, director of the Center for Cellular Immunotherapies, and director of the Parker Institute for Cancer Therapy at the University of Pennsylvania.

VIEW THE AGENDA AND REGISTER

Know someone who’d like to attend? Forward to a friend.

The Gladstone-UCSF Institute of Genomic Immunology was launched in 2020 to bring experts in diverse, rapidly advancing fields together around the shared goal of understanding how to genetically control human immune cells and using this knowledge to develop innovative cell‑based immunotherapies.

View in Your Browser

© 2021 Gladstone Institutes. All rights reserved.

Want to change how you receive these emails?
Update your preferences or unsubscribe from this list.

Gladstone Institutes
1650 Owens Street
San Francisco, CA  94158

Add us to your address book

Wed, Oct 20, 12:59 PM (6 days ago)
to me

Monday, October 25, 2021

Hear from Carl June at the Gladstone-UCSF Institute of Genomic Immunology Symposium Inbox Gladstone Events Unsubscribe Wed, Oct 20, 12:59 PM (5 days ago) to me Images are not displayed. Display images below - Always display images from events@gladstone.ucsf.edu Forward to a Friend GLADSTONE-UCSF INSTITUTE OF GENOMIC IMMUNOLOGY Innovators in Genomic Immunology November 3, 2021 9am–5pm PDT • Online Join this 1-day symposium to meet the investigators of the Gladstone-UCSF Institute of Genomic Immunology and discover how their labs are using genomic and genome engineering technologies to address key questions in human immunology and repurpose the immune system to fight disease. You’ll also get to hear from invited speaker Carl June, director of the Center for Cellular Immunotherapies, and director of the Parker Institute for Cancer Therapy at the University of Pennsylvania. VIEW THE AGENDA AND REGISTER Know someone who’d like to attend? Forward to a friend. The Gladstone-UCSF Institute of Genomic Immunology was launched in 2020 to bring experts in diverse, rapidly advancing fields together around the shared goal of understanding how to genetically control human immune cells and using this knowledge to develop innovative cell‑based immunotherapies. View in Your Browser © 2021 Gladstone Institutes. All rights reserved. Want to change how you receive these emails? Update your preferences or unsubscribe from this list. Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 Add us to your address book

Sunday, October 10, 2021

FREEDOM, SEX AND TERRITORY.

FREEDOM, SEX AND TERRITORY:

Tools to Investigate SARS-CoV-2 Infection

GENTLE PEOPLE:

 It has been over a year now since the covid-19 virus created an international pandemic of sickness and death. As of this date, Sunday, October 10, we continue to live in fear of the deadly virus but thanks to new and quickly developed and distributed vaccines, millions of us have survived the pandemic.

THE BIG QUESTION NOW IS, ARE WE FINALLY SAFE? 

SADLY, THE ANSWER IS NO! THE VIRUS HAS MUTATED AND WE NEED TO CONTINUE DEVELOPING NEW AND BETTER METHODS TO BLOCK COVID AND OTHER DANGEROUS VIRUS FROM PROLIFERATING...

COVID-19 is caused by the coronavirus SARS CoV-2, previously called 2019-nCoV. It belongs to the Coronaviridae family and is broadly distributed in humans and other mammals. hCoV-229E, OC43, NL63 and HKU1 are some of the known coronaviruses that cause mild respiratory diseases unlike SAR-CoV and MERS that cause severe to fatal respiratory diseases [1].

Why is SARS-CoV-2 spreading faster than its two ancestors? Why is SARS-CoV-2 lethal? Recent publications have shown that there are differences in their genome structure and immunological response to SARS-CoV-2 infection. The key markers involved in these interactions include Spike protein (S), Nucleocapsid (N), ACE-2 receptor, FURIN protease in addition to the cytokines.

Image 1: Coronavirus with Spike Image
Coronavirus with Spike

Spike Protein (S): SARS-CoV2 enters the cells through the Spike mediated interaction with the ECD domain of the ACE2 cell receptor. (A recombinant fusion protein (RBD of Spike protein and ECD of membrane protein) can be a great tool to investigate this interaction.

Learn more about the purified recombinant Spike Protein (S).


Image 2: TMPRSS2 Image
ACE2 and TMPRSS2 in Coronoa virus

Transmembrane Serine Protease 2 (TMPRSS2): This is a serine protease that cleaves and activates the viral spike glycoproteins which facilitates virus-cell membrane fusions. A recent study showed that SARS-CoV-2 needs both ACE2 receptor and serine protease TMPRSS2 for protein priming to enter the cell.[2]

Tools for analyzing TMPRSS2

  1. ORF clones
  2. Goat and Rabbit Polyclonal Antibodies
  3. CRISPR kits
  4. Unique controls

Image 3: Nucleocapsid NC Image
Corona virus structure 

Nucleocapsid: The nucleocapsid phosphoprotein packages the viral genome into a helical ribonucelocapsid, thus playing a crucial role in viral self-assembly.

Tools for analyzing the Nucleocapsid

  1. Recombinant SARS-CoV-2 Nucleocapsid Protein
  2. Biotinylated recombinant SARS-CoV-2 Nucleocapsid protein
  3. SARS-CoV-2 N protein Rabbit Polyclonal Antibody

Image 4: ACE-2 Receptor Protein Image
ACE-2 Receptor Protein

ACE-2 receptor: ACE-2 is the host cell receptor responsible for mediating infection by SARS-CoV-2.

Tools for analyzing ACE-2 protein

  1. Human Recombinant Protein Angiotensin Converting Enzyme (ACE2)
  2. ACE-2 specific Mouse Monoclonal Antibody
  3. ACE-2 Human ORF clones

Find out more about the additional ACE-2 specific tools here


Image 5: Furin Protein Image
ACE-2 Receptor Protein 

Furin: It is a protease present in many human organs that recognizes and activates a specific site on the SARS-CoV-2 Spike protein, thus facilitating a tighter binding to the ACE-2 receptor and might play a role in the higher infection rate [3].

How can you analyze Furin? Learn about the tools for analyzing Furin.


Image 6: IL2 Pathway Image
IL2 Pathway

Cytokines: Studies have shown a strong correlation between severity of the disease and concentrations of IL2, IL7, IL10, GCSF, MCP1 and TNF alpha [1].

Tools to analyze the 6 cytokines associated Cytokine Storm:
IL2 | IL7 | IL10 | GCSF | MCP1 | TNF alpha

References:

  1. https://link.springer.com/article/10.1007/s12098-020-03263-6
  2. https://science.sciencemag.org/content/367/6483/1260
  3. https://science.sciencemag.org/content/367/6483/1260

Monday, October 4, 2021

 

ATTENTION WORLD GOVERNMENT LEADERS!

 YOUR SCHOOLS ARE CURRENTLY TRAINING CHILDREN TO WORK FOR BIG BUSINESS. THIS MUST STOP! CHILDREN NEED TO CHANGE AND GREEN THE ECONOMY TO HELP PRESERVE NATURE. WE NEED AN ECONOMY BASED ON NATURE AND NOT AN ECONOMY BASED ON OIL AND GAS AND POLLUTING INDUSTRY.

 EVERY SCHOOL MUST HAVE : TREES, VEGETABLES, AND FLOWER GARDENS, WITH CHILDREN LEARNING ABOUT AND CARING FOR PLANTS. WHY? BECAUSE WITHOUT NATURE LIFE CEASES TO EXIST AND TODAY NATURE IS BARELY SUSTAINING THE LIFE OUR POLLUTING INDUSTRIAL ECONOMY IS DESTROYING!  THIS IS NOT AN OPINION OR A THEORY, IT IS A FACT.

WE NEED PEOPLE WHO HAVE LEARNED IN CHILDHOOD AS MUCH BOTANY AND BIOLOGY AND  WAYS TO PROTECT THE ENVIRONMENT AS IS HUMANLY POSSIBLE AND WHO ARE NOT AFRAID TO TAKE ACTION.  IT BEGINS BY CHANGING THE SCHOOL ENVIRONMENT AND IT ENDS WITH THE CREATION OF A BEAUTIFUL NATURAL ENVIRONMENT WE CAN ALL BE PROUD OF.

N.J.R.

THE FOLLOWING ARE MORALLY WRONG AND ETHICALLY EVIL...

1. THE MURDER OF  AIR BREATHING FRIENDLY DOLPHINS AND WHALES FOR THEIR MEAT AND OIL.

2. THE POLLUTION OF AIR AND WATER WITH OIL AND PLASTIC AND GAS.

3. GENETICALLY CONVERTED SEEDS THAT RESIST POLLUTION AND PESTICIDES AND ALLOW FARMERS THE USE OF DANGEROUS PESTICIDES WHICH CONSEQUENTLY KILL SONG BIRDS AND BEES.

  Hello my good friend Valdemar Oliveira! I am happy to hear you had a successfull heart operation.  I hope you live to be 110. I may not be...